John R. Engen

Research interests

Publications

• Advances in Hydrogen/Deuterium Exchange Mass Spectrometry and the Pursuit of Challenging Biological Systems

  Chemical Reviews · 2021 · https://doi.org/10.1021/acs.chemrev.1c00279

• Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice

  Nature Biotechnology · 2019 · https://doi.org/10.1038/s41587-019-0222-z

• Substrate processing by the Cdc48 ATPase complex is initiated by ubiquitin unfolding

  Science · 2019 · https://doi.org/10.1126/science.aax1033

• Tissue-Specific Oncogenic Activity of KRASA146T

  Cancer Discovery · 2019 · https://doi.org/10.1158/2159-8290.cd-18-1220

• Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments

  Nature Methods · 2019 · https://doi.org/10.1038/s41592-019-0459-y

• Chemically Induced Degradation of the Oncogenic Transcription Factor BCL6

  Cell Reports · 2017 · https://doi.org/10.1016/j.celrep.2017.08.081

• Force interacts with macromolecular structure in activation of TGF-β

  Nature · 2017 · https://doi.org/10.1038/nature21035

• Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome

  Science · 2016 · https://doi.org/10.1126/science.aad9421

• Therapeutic Targeting of Oncogenic K‐Ras by a Covalent Catalytic Site Inhibitor

  Angewandte Chemie International Edition · 2013 · https://doi.org/10.1002/anie.201307387

• Hydrogen/deuterium exchange mass spectrometry for probing higher order structure of protein therapeutics: methodology and applications

  Drug Discovery Today · 2013 · https://doi.org/10.1016/j.drudis.2013.07.019

• Catalytic site remodelling of the DOT1L methyltransferase by selective inhibitors

  Nature Communications · 2012 · https://doi.org/10.1038/ncomms2304

• Analytical tools for characterizing biopharmaceuticals and the implications for biosimilars

  Nature Reviews Drug Discovery · 2012 · https://doi.org/10.1038/nrd3746

• Targeting Bcr–Abl by combining allosteric with ATP-binding-site inhibitors

  Nature · 2010 · https://doi.org/10.1038/nature08675

• The Utility of Hydrogen/Deuterium Exchange Mass Spectrometry in Biopharmaceutical Comparability Studies

  Journal of Pharmaceutical Sciences · 2010 · https://doi.org/10.1002/jps.22432

• Post-translational Modifications Differentially Affect IgG1 Conformation and Receptor Binding

  Molecular & Cellular Proteomics · 2010 · https://doi.org/10.1074/mcp.m900540-mcp200

• Analysis of Protein Conformation and Dynamics by Hydrogen/Deuterium Exchange MS

  Analytical Chemistry · 2009 · https://doi.org/10.1021/ac901154s

• Novel mutant-selective EGFR kinase inhibitors against EGFR T790M

  Nature · 2009 · https://doi.org/10.1038/nature08622

• Characterization of IgG1 Conformation and Conformational Dynamics by Hydrogen/Deuterium Exchange Mass Spectrometry

  Analytical Chemistry · 2009 · https://doi.org/10.1021/ac802575y

• High-Speed and High-Resolution UPLC Separation at Zero Degrees Celsius

  Analytical Chemistry · 2008 · https://doi.org/10.1021/ac8008862

• Semi-automated data processing of hydrogen exchange mass spectra using HX-Express

  Journal of the American Society for Mass Spectrometry · 2006 · https://doi.org/10.1016/j.jasms.2006.07.025

• Identification and characterization of EX1 kinetics in H/D exchange mass spectrometry by peak width analysis

  Journal of the American Society for Mass Spectrometry · 2006 · https://doi.org/10.1016/j.jasms.2006.05.014

• Hydrogen exchange mass spectrometry for the analysis of protein dynamics

  Mass Spectrometry Reviews · 2005 · https://doi.org/10.1002/mas.20064

• Peer Reviewed: Investigating Protein Structure and Dynamics by Hydrogen Exchange MS

  Analytical Chemistry · 2001 · https://doi.org/10.1021/ac012452f

Current projects

No projects listed.