Roberto Fernández‐Lafuente

Research interests

Publications

• Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization

  Chemical Society Reviews · 2022 · 10.1039/d2cs00083k

• Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies

  Biotechnology Advances · 2021 · 10.1016/j.biotechadv.2021.107821

• Use of Alcalase in the production of bioactive peptides: A review

  International Journal of Biological Macromolecules · 2020 · 10.1016/j.ijbiomac.2020.10.060

• Novozym 435: the “perfect” lipase immobilized biocatalyst?

  Catalysis Science & Technology · 2019 · 10.1039/c9cy00415g

• Parameters necessary to define an immobilized enzyme preparation

  Process Biochemistry · 2019 · 10.1016/j.procbio.2019.11.026

• Immobilization of lipases on hydrophobic supports: immobilization mechanism, advantages, problems, and solutions

  Biotechnology Advances · 2019 · 10.1016/j.biotechadv.2019.04.003

• Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts

  Biotechnology Advances · 2015 · https://doi.org/10.1016/j.biotechadv.2015.03.006

• Importance of the Support Properties for Immobilization or Purification of Enzymes

  ChemCatChem · 2015 · 10.1002/cctc.201500310

• Immobilization of lipases on hydrophobic supports involves the open form of the enzyme

  Enzyme and Microbial Technology · 2015 · 10.1016/j.enzmictec.2015.02.001

• Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization

  RSC Advances · 2013 · https://doi.org/10.1039/c3ra45991h

• Heterofunctional Supports in Enzyme Immobilization: From Traditional Immobilization Protocols to Opportunities in Tuning Enzyme Properties

  Biomacromolecules · 2013 · 10.1021/bm400762h

• Modifying enzyme activity and selectivity by immobilization

  Chemical Society Reviews · 2012 · https://doi.org/10.1039/c2cs35231a

• Enhancing the functional properties of thermophilic enzymes by chemical modification and immobilization

  Enzyme and Microbial Technology · 2011 · 10.1016/j.enzmictec.2011.06.023

• Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance

  Advanced Synthesis & Catalysis · 2011 · https://doi.org/10.1002/adsc.201100534

• Coupling Chemical Modification and Immobilization to Improve the Catalytic Performance of Enzymes

  Advanced Synthesis & Catalysis · 2011 · 10.1002/adsc.201100163

• Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance

  Enzyme and Microbial Technology · 2010 · 10.1016/j.enzmictec.2010.10.003

• Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation

  Enzyme and Microbial Technology · 2009 · 10.1016/j.enzmictec.2009.08.009

• Lipase from Thermomyces lanuginosus: Uses and prospects as an industrial biocatalyst

  Journal of Molecular Catalysis B Enzymatic · 2009 · 10.1016/j.molcatb.2009.11.010

• Improvement of enzyme activity, stability and selectivity via immobilization techniques

  Enzyme and Microbial Technology · 2007 · https://doi.org/10.1016/j.enzmictec.2007.01.018

• Different mechanisms of protein immobilization on glutaraldehyde activated supports: Effect of support activation and immobilization conditions

  Enzyme and Microbial Technology · 2006 · 10.1016/j.enzmictec.2006.01.014

• Glyoxyl agarose: A fully inert and hydrophilic support for immobilization and high stabilization of proteins

  Enzyme and Microbial Technology · 2005 · 10.1016/j.enzmictec.2005.10.014

• Interfacial adsorption of lipases on very hydrophobic support (octadecyl–Sepabeads): immobilization, hyperactivation and stabilization of the open form of lipases

  Journal of Molecular Catalysis B Enzymatic · 2002 · 10.1016/s1381-1177(02)00178-9

• Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment☆

  Enzyme and Microbial Technology · 2000 · 10.1016/s0141-0229(99)00188-x

• Immobilization of lipases by selective adsorption on hydrophobic supports

  Chemistry and Physics of Lipids · 1998 · 10.1016/s0009-3084(98)00042-5

• A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports

  Biotechnology and Bioengineering · 1998 · 10.1002/(sici)1097-0290(19980605)58:5<486::aid-bit4>3.0.co;2-9

Current projects

No projects listed.