James M. Holton

Research interests

Publications

• Large language models generate functional protein sequences across diverse families

  Nature Biotechnology · 2023 · https://doi.org/10.1038/s41587-022-01618-2

• Fragment binding to the Nsp3 macrodomain of SARS-CoV-2 identified through crystallographic screening and computational docking

  Science Advances · 2021 · https://doi.org/10.1126/sciadv.abf8711

• Untangling the sequence of events during the S 2 → S 3 transition in photosystem II and implications for the water oxidation mechanism

  Proceedings of the National Academy of Sciences · 2020 · https://doi.org/10.1073/pnas.2000529117

• Structures of the intermediates of Kok’s photosynthetic water oxidation clock

  Nature · 2018 · https://doi.org/10.1038/s41586-018-0681-2

• Guide-bound structures of an RNA-targeting A-cleaving CRISPR–Cas13a enzyme

  Nature Structural & Molecular Biology · 2017 · 10.1038/nsmb.3466

• Guide-bound structures of an RNA-targeting A-cleaving CRISPR–Cas13a enzyme

  Nature Structural & Molecular Biology · 2017 · 10.1038/nsmb.3466

• Mapping the conformational landscape of a dynamic enzyme by multitemperature and XFEL crystallography

  eLife · 2015 · 10.7554/elife.07574

• Mapping the conformational landscape of a dynamic enzyme by multitemperature and XFEL crystallography

  eLife · 2015 · 10.7554/elife.07574

• Implementation and performance of SIBYLS: a dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the Advanced Light Source

  Journal of Applied Crystallography · 2013 · https://doi.org/10.1107/s0021889812048698

• Time-resolved protein nanocrystallography using an X-ray free-electron laser

  Optics Express · 2012 · https://doi.org/10.1364/oe.20.002706

• Femtosecond X-ray protein nanocrystallography

  Nature · 2011 · https://doi.org/10.1038/nature09750

• Accessing protein conformational ensembles using room-temperature X-ray crystallography

  Proceedings of the National Academy of Sciences · 2011 · https://doi.org/10.1073/pnas.1111325108

• Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser

  Physical Review B · 2011 · 10.1103/physrevb.84.214111

• Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser

  Physical Review B · 2011 · 10.1103/physrevb.84.214111

• Femtosecond protein nanocrystallography—data analysis methods

  Optics Express · 2010 · 10.1364/oe.18.005713

• The minimum crystal size needed for a complete diffraction data set

  Acta Crystallographica Section D Biological Crystallography · 2010 · 10.1107/s0907444910007262

• Femtosecond protein nanocrystallography—data analysis methods

  Optics Express · 2010 · 10.1364/oe.18.005713

• Automated electron‐density sampling reveals widespread conformational polymorphism in proteins

  Protein Science · 2010 · 10.1002/pro.423

• The minimum crystal size needed for a complete diffraction data set

  Acta Crystallographica Section D Biological Crystallography · 2010 · 10.1107/s0907444910007262

• Automated electron‐density sampling reveals widespread conformational polymorphism in proteins

  Protein Science · 2010 · 10.1002/pro.423

• A beginner's guide to radiation damage

  Journal of Synchrotron Radiation · 2009 · https://doi.org/10.1107/s0909049509004361

• An assessment of the resolution limitation due to radiation-damage in X-ray diffraction microscopy

  Journal of Electron Spectroscopy and Related Phenomena · 2008 · https://doi.org/10.1016/j.elspec.2008.10.008

• Structural basis for aminoglycoside inhibition of bacterial ribosome recycling

  Nature Structural & Molecular Biology · 2007 · https://doi.org/10.1038/nsmb1271

• Basis for a ubiquitin-like protein thioester switch toggling E1–E2 affinity

  Nature · 2007 · 10.1038/nature05490

• Basis for a ubiquitin-like protein thioester switch toggling E1–E2 affinity

  Nature · 2007 · 10.1038/nature05490

• Structural Basis for Recruitment of Ubc12 by an E2 Binding Domain in NEDD8's E1

  Molecular Cell · 2005 · 10.1016/j.molcel.2004.12.020

• Structural and mechanistic studies of VPS4 proteins

  The EMBO Journal · 2005 · 10.1038/sj.emboj.7600818

• Structures of the Bacterial Ribosome at 3.5 A Resolution

  Science · 2005 · https://doi.org/10.1126/science.1117230

• Structural Basis for Recruitment of Ubc12 by an E2 Binding Domain in NEDD8's E1

  Molecular Cell · 2005 · 10.1016/j.molcel.2004.12.020

• Structural and mechanistic studies of VPS4 proteins

  The EMBO Journal · 2005 · 10.1038/sj.emboj.7600818

• Automated protein crystal structure determination using elves

  Proceedings of the National Academy of Sciences · 2004 · 10.1073/pnas.0306241101

• Ubiquitin Recognition by the Human TSG101 Protein

  Molecular Cell · 2004 · 10.1016/s1097-2765(04)00129-7

• Automated protein crystal structure determination using elves

  Proceedings of the National Academy of Sciences · 2004 · 10.1073/pnas.0306241101

• Ubiquitin Recognition by the Human TSG101 Protein

  Molecular Cell · 2004 · 10.1016/s1097-2765(04)00129-7

• The Structure of the APPBP1-UBA3-NEDD8-ATP Complex Reveals the Basis for Selective Ubiquitin-like Protein Activation by an E1

  Molecular Cell · 2003 · https://doi.org/10.1016/s1097-2765(03)00452-0

• Crystallographic analysis of CD40 recognition and signaling by human TRAF2

  Proceedings of the National Academy of Sciences · 1999 · 10.1073/pnas.96.15.8408

• Crystallographic analysis of CD40 recognition and signaling by human TRAF2

  Proceedings of the National Academy of Sciences · 1999 · 10.1073/pnas.96.15.8408

Current projects

No projects listed.