Mark Howarth

Research interests

Publications

• Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice

  Science · 2021 · 10.1126/science.abf6840

• A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses

  Nature Communications · 2021 · 10.1038/s41467-020-20654-7

• Power to the protein: enhancing and combining activities using the Spy toolbox

  Chemical Science · 2020 · 10.1039/d0sc01878c

• Approaching infinite affinity through engineering of peptide–protein interaction

  Proceedings of the National Academy of Sciences · 2019 · 10.1073/pnas.1909653116

• Engineering a Rugged Nanoscaffold To Enhance Plug-and-Display Vaccination

  ACS Nano · 2018 · 10.1021/acsnano.8b02805

• Evolving Accelerated Amidation by SpyTag/SpyCatcher to Analyze Membrane Dynamics

  Angewandte Chemie International Edition · 2017 · 10.1002/anie.201707623

• Plug-and-Display: decoration of Virus-Like Particles via isopeptide bonds for modular immunization

  Scientific Reports · 2016 · 10.1038/srep19234

• Programmable polyproteams built using twin peptide superglues

  Proceedings of the National Academy of Sciences · 2016 · 10.1073/pnas.1519214113

• Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher

  Current Opinion in Chemical Biology · 2015 · 10.1016/j.cbpa.2015.10.002

• SpyLigase peptide–peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture

  Proceedings of the National Academy of Sciences · 2014 · 10.1073/pnas.1315776111

• Site-Specific Biotinylation of Purified Proteins Using BirA

  Methods in molecular biology · 2014 · 10.1007/978-1-4939-2272-7_12

• SpyTag/SpyCatcher Cyclization Confers Resilience to Boiling on a Mesophilic Enzyme

  Angewandte Chemie International Edition · 2014 · 10.1002/anie.201402519

• Structural Analysis and Optimization of the Covalent Association between SpyCatcher and a Peptide Tag

  Journal of Molecular Biology · 2013 · 10.1016/j.jmb.2013.10.021

• Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin

  Proceedings of the National Academy of Sciences · 2012 · https://doi.org/10.1073/pnas.1115485109

• How the biotin–streptavidin interaction was made even stronger: investigation via crystallography and a chimaeric tetramer

  Biochemical Journal · 2011 · 10.1042/bj20101593

• Type 1 Insulin-like Growth Factor Receptor Translocates to the Nucleus of Human Tumor Cells

  Cancer Research · 2010 · 10.1158/0008-5472.can-10-0052

• A streptavidin variant with slower biotin dissociation and increased mechanostability

  Nature Methods · 2010 · 10.1038/nmeth.1450

• Compact Biocompatible Quantum Dots Functionalized for Cellular Imaging

  Journal of the American Chemical Society · 2008 · 10.1021/ja076069p

• Monovalent, reduced-size quantum dots for imaging receptors on living cells

  Nature Methods · 2008 · 10.1038/nmeth.1206

• Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin

  Nature Protocols · 2008 · 10.1038/nprot.2008.20

• A monovalent streptavidin with a single femtomolar biotin binding site

  Nature Methods · 2006 · 10.1038/nmeth861

• Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase

  Nature Methods · 2005 · 10.1038/nmeth735

• Targeting quantum dots to surface proteins in living cells with biotin ligase

  Proceedings of the National Academy of Sciences · 2005 · 10.1073/pnas.0503125102

• Tapasin enhances MHC class I peptide presentation according to peptide half-life

  Proceedings of the National Academy of Sciences · 2004 · 10.1073/pnas.0306294101

• Assembly and Antigen-Presenting Function of MHC Class I Molecules in Cells Lacking the ER Chaperone Calreticulin

  Immunity · 2002 · 10.1016/s1074-7613(01)00260-6

Current projects

No projects listed.