Michael W. Pfaffl

Research interests

Publications

• MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines

  Clinical Chemistry · 2025 · 10.1093/clinchem/hvaf043

• MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines

  Clinical Chemistry · 2025 · 10.1093/clinchem/hvaf043

• The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020

  Clinical Chemistry · 2020 · https://doi.org/10.1093/clinchem/hvaa125

• Evaluation of serum extracellular vesicle isolation methods for profiling miRNAs by next‐generation sequencing

  Journal of Extracellular Vesicles · 2018 · 10.1080/20013078.2018.1481321

• Evaluation of serum extracellular vesicle isolation methods for profiling miRNAs by next‐generation sequencing

  Journal of Extracellular Vesicles · 2018 · 10.1080/20013078.2018.1481321

• EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research

  Nature Methods · 2017 · https://doi.org/10.1038/nmeth.4185

• Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper

  Journal of Extracellular Vesicles · 2017 · https://doi.org/10.1080/20013078.2017.1286095

• How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments

  Biomolecular Detection and Quantification · 2015 · 10.1016/j.bdq.2015.01.005

• How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments

  Biomolecular Detection and Quantification · 2015 · 10.1016/j.bdq.2015.01.005

• The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

  Clinical Chemistry · 2013 · https://doi.org/10.1373/clinchem.2013.206375

• The need for transparency and good practices in the qPCR literature

  Nature Methods · 2013 · https://doi.org/10.1038/nmeth.2697

• Evaluation of qPCR curve analysis methods for reliable biomarker discovery: Bias, resolution, precision, and implications

  Methods · 2012 · 10.1016/j.ymeth.2012.08.011

• Evaluation of qPCR curve analysis methods for reliable biomarker discovery: Bias, resolution, precision, and implications

  Methods · 2012 · 10.1016/j.ymeth.2012.08.011

• RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalization

  BMC Genomics · 2011 · 10.1186/1471-2164-12-156

• RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalization

  BMC Genomics · 2011 · 10.1186/1471-2164-12-156

• mRNA and microRNA quality control for RT-qPCR analysis

  Methods · 2010 · 10.1016/j.ymeth.2010.01.010

• Normalization strategies for microRNA profiling experiments: a ‘normal’ way to a hidden layer of complexity?

  Biotechnology Letters · 2010 · 10.1007/s10529-010-0380-z

• mRNA and microRNA quality control for RT-qPCR analysis

  Methods · 2010 · 10.1016/j.ymeth.2010.01.010

• Normalization strategies for microRNA profiling experiments: a ‘normal’ way to a hidden layer of complexity?

  Biotechnology Letters · 2010 · 10.1007/s10529-010-0380-z

• The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

  Clinical Chemistry · 2009 · https://doi.org/10.1373/clinchem.2008.112797

• Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR

  Biotechnology Letters · 2006 · 10.1007/s10529-006-9127-2

• RNA integrity and the effect on the real-time qRT-PCR performance

  Molecular Aspects of Medicine · 2006 · https://doi.org/10.1016/j.mam.2005.12.003

• Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR

  Biotechnology Letters · 2006 · 10.1007/s10529-006-9127-2

• Quantitative real-time RT-PCR – a perspective

  Journal of Molecular Endocrinology · 2005 · 10.1677/jme.1.01755

• Quantitative real-time RT-PCR – a perspective

  Journal of Molecular Endocrinology · 2005 · 10.1677/jme.1.01755

• Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations

  Biotechnology Letters · 2004 · https://doi.org/10.1023/b:bile.0000019559.84305.47

• Comparison of Reverse Transcriptases in Gene Expression Analysis

  Clinical Chemistry · 2004 · 10.1373/clinchem.2004.035469

• Comparison of Reverse Transcriptases in Gene Expression Analysis

  Clinical Chemistry · 2004 · 10.1373/clinchem.2004.035469

• Short-term changes of mRNA expression of various inflammatory factors and milk proteins in mammary tissue during LPS-induced mastitis

  Domestic Animal Endocrinology · 2003 · 10.1016/j.domaniend.2003.09.003

• Short-term changes of mRNA expression of various inflammatory factors and milk proteins in mammary tissue during LPS-induced mastitis

  Domestic Animal Endocrinology · 2003 · 10.1016/j.domaniend.2003.09.003

• Real-time RT-PCR quantification of insulin-like growth factor (IGF)-1, IGF-1 receptor, IGF-2, IGF-2 receptor, insulin receptor, growth hormone receptor, IGF-binding proteins 1, 2 and 3 in the bovine species

  Domestic Animal Endocrinology · 2002 · 10.1016/s0739-7240(01)00128-x

• Real-time RT-PCR quantification of insulin-like growth factor (IGF)-1, IGF-1 receptor, IGF-2, IGF-2 receptor, insulin receptor, growth hormone receptor, IGF-binding proteins 1, 2 and 3 in the bovine species

  Domestic Animal Endocrinology · 2002 · 10.1016/s0739-7240(01)00128-x

• Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCR

  Nucleic Acids Research · 2002 · https://doi.org/10.1093/nar/30.9.e36

• Validities of mRNA quantification using recombinant RNA and recombinant DNA external calibration curves in real-time RT-PCR

  Biotechnology Letters · 2001 · 10.1023/a:1005658330108

• Validities of mRNA quantification using recombinant RNA and recombinant DNA external calibration curves in real-time RT-PCR

  Biotechnology Letters · 2001 · 10.1023/a:1005658330108

• A new mathematical model for relative quantification in real-time RT-PCR

  Nucleic Acids Research · 2001 · https://doi.org/10.1093/nar/29.9.e45

Current projects

No projects listed.