Stephen G. Withers

Research interests

Publications

• Carbohydrate-active enzymes (CAZymes) in the gut microbiome

  Nature Reviews Microbiology · 2022 · https://doi.org/10.1038/s41579-022-00712-1

• Alternative Catalytic Anions Differentially Modulate Human α-Amylase Activity and Specificity,

  Biochemistry · 2008 · https://doi.org/10.1021/bi701652t

• Engineering of glycosidases and glycosyltransferases

  Current Opinion in Chemical Biology · 2006 · https://doi.org/10.1016/j.cbpa.2006.07.015

• High-throughput screening methodology for the directed evolution of glycosyltransferases

  Nature Methods · 2006 · https://doi.org/10.1038/nmeth899

• Structural Insights into the Catalytic Mechanism of Trypanosoma cruzi trans-Sialidase

  Structure · 2004 · https://doi.org/10.1016/j.str.2004.02.036

• Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog

  Nature Structural & Molecular Biology · 2004 · https://doi.org/10.1038/nsmb720

• A Structural View of the Action ofEscherichia coli(lacZ) β-Galactosidase,

  Biochemistry · 2001 · https://doi.org/10.1021/bi011727i

• Crystal structure of the retaining galactosyltransferase LgtC from Neisseria meningitidis in complex with donor and acceptor sugar analogs.

  Nature Structural Biology · 2001 · https://doi.org/10.1038/84168

• Crystallographic Evidence for Substrate-assisted Catalysis in a Bacterial β-Hexosaminidase

  Journal of Biological Chemistry · 2001 · https://doi.org/10.1074/jbc.m011067200

• Catalysis by hen egg-white lysozyme proceeds via a covalent intermediate

  Nature · 2001 · https://doi.org/10.1038/35090602

• Glycosyl fluorides in enzymatic reactions

  Carbohydrate Research · 2000 · https://doi.org/10.1016/s0008-6215(00)00041-0

• Glycosidase mechanisms: anatomy of a finely tuned catalyst.

  Accounts of Chemical Research · 2000 · https://doi.org/10.1021/ar970172+

• Glycosidase mechanisms

  Current Opinion in Chemical Biology · 2000 · https://doi.org/10.1016/s1367-5931(00)00135-6

• Subsite Mapping of the Human Pancreatic α-Amylase Active Site through Structural, Kinetic, and Mutagenesis Techniques,

  Biochemistry · 2000 · https://doi.org/10.1021/bi9921182

• Hydrogen bonding and catalysis: a novel explanation for how a single amino acid substitution can change the ph optimum of a glycosidase 1 1Edited by M. F. Summers

  Journal of Molecular Biology · 2000 · https://doi.org/10.1006/jmbi.2000.3722

• X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the α-amylase family

  Nature Structural Biology · 1999 · https://doi.org/10.1038/8235

• Mutagenesis of Glycosidases

  Annual Review of Biochemistry · 1999 · https://doi.org/10.1146/annurev.biochem.68.1.487

• Glycosynthases: Mutant Glycosidases for Oligosaccharide Synthesis

  Journal of the American Chemical Society · 1998 · https://doi.org/10.1021/ja980833d

• Snapshots along an Enzymatic Reaction Coordinate: Analysis of a Retaining β-Glycoside Hydrolase,

  Biochemistry · 1998 · https://doi.org/10.1021/bi981315i

• The p K a of the General Acid/Base Carboxyl Group of a Glycosidase Cycles during Catalysis: A 13 C-NMR Study of Bacillus circulans Xylanase

  Biochemistry · 1996 · https://doi.org/10.1021/bi9613234

• NAG-thiazoline, An N-Acetyl-β-hexosaminidase Inhibitor That Implicates Acetamido Participation

  Journal of the American Chemical Society · 1996 · https://doi.org/10.1021/ja960826u

• The structure of human pancreatic α -amylase at 1.8 Å resolution and comparisons with related enzymes

  Protein Science · 1995 · https://doi.org/10.1002/pro.5560040908

• Stereoselective hydrolysis catalyzed by related beta-1,4-glucanases and beta-1,4-xylanases.

  Journal of Biological Chemistry · 1992 · https://doi.org/10.1016/s0021-9258(18)42313-7

• Mechanism of Agrobacterium .beta.-glucosidase: kinetic studies

  Biochemistry · 1992 · https://doi.org/10.1021/bi00156a015

• Unequivocal demonstration of the involvement of a glutamate residue as a nucleophile in the mechanism of a retaining glycosidase

  Journal of the American Chemical Society · 1990 · https://doi.org/10.1021/ja00171a043

Current projects

No projects listed.